Regulation of aspartokinase III synthesis in Escherichia coli: isolation of mutants containing lysC-lac fusions.
نویسندگان
چکیده
Mutants containing fusions of the lac gene to the lysC gene were isolated. In these, the expression of beta-galactosidase was regulated by lysine (and arginine), as previously described for aspartokinase III.
منابع مشابه
The leader sequence of the Escherichia coli lysC gene is involved in the regulation of LysC synthesis.
In Escherichia coli and Bacillus subtilis, long leader sequences are found upstream of the lysC coding sequences which encode lysine-sensitive aspartokinase. Highly conserved regions exist between these sequences. Mutations leading to constitutive expression of the E. coli lysC gene have been localised within these conserved regions, indicating that they participate in the lysine-mediated repre...
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Aspartokinase III (AKIII), one of three isozymes of EScherichia coti K-12, is iilhibited al]esterically by L-lysine. This enzyme is encoded by the lysC gene and has 449 amino acid residues. We analyzed the feedback inhibition site of AKIII by generating yarious 4)ngC mutants in a plasmid yector. These mutants conferred resistance to L-lysine and!or an L-lysine analogue on their host. The inhibi...
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عنوان ژورنال:
- Journal of bacteriology
دوره 143 1 شماره
صفحات -
تاریخ انتشار 1980